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Transfection MS751 and C33A cells were transfected with various plasmids using lipofectamine 2000 (Invitrogen) according to the vendors instructions

Two transcription factors that form an integral part of the pathways augmented during this transition period are HIF-1 and NF-B, both of which are sufficiently tuned to govern a specific response in hypoxia and a relatively hyperoxic shift [184191]

Glycine and serine (serine is converted to glycine for GSH synthesis) are spent making GSH, but glutamate is increased once GSH exits the cell and is transaminated by GGT, releasing glutamate into circulation

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Medical use of cannabis for glaucoma

We observed good correspondence between the relative and absolute GSH levels (Figure 3), and the statistical significance of GSH level changes with time post dose was the same regardless of the quantitation approach